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Westerns are one of the most utilized tools in biology. There are several steps you have to take before you can actually perform a western. 1. Run experiment and collect samples (break open). 2. Lyse the cells to extract proteins. 3. Run proteins on a gel (most commonly this is an SDS PAGE gel when you only care about the size of a protein) 4. Transfer proteins to a memebane. 5. Western (probe for the protein with an antibody). #phdlife #westernblot #westernblotting #biology #molecularbiology #g
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